Perbedaan Gambaran Histologi kelenjar Timus Kelinci Muda dan Kelinci Tua Setelah Pemberian Suntikan Prostaglandin (PGF 2A)

Tahun 1997 Volume 32 Nomor 1
Oleh : Nurjaman

Thymus gland is the important organ in immunity, especially in producing lymphocyte T cells. Prostaglandin is an antigenic substance that functions as a mitogen.

The aim of this to determine the influence of Prostaglandin as mitogen on the thymus gland of aged male rabbits and young male rabbits.

Forty male rabbits were divided into two groups, the firs group consists of twenty aged male rabbits and the second group consists of twenty young rabbits . The twenty aged rabbits were divided randomly into two groups (group I = 10 rabbits, group II = 10 rabbits). The twenty young rabbits were divided randomly into two groups (group I = 10 rabbits, group II = 10 rabbits). Before the experiment began, all rabbits were observed for one week for adaptation. After adaptation all rabbits were injected with prostaglandin intra musculary, 1.5 mg for each aged rabbit and 0.75 mg for each young rabbit. One week after injection all rabbits of group I (aged and young rabbits) were killed and the thymus gland fixated in Bouin solution for three hours and than in 10% formalin solution for twenty hours. At the next stage the thymus glands were processed by microtechnical procedure, paraffin methods and haematoxyllin eosin staining. Those preparations were examined by light microscope. Two weeks after injection all rabbits in group II (aged and young rabbits) were killed. The thymus glands were processed as the group I

The reaction of the thymus glands after injection with prostaglandin was proliferation of lymphocyte cells. Proliferation of the lymphocyte cells in the thymus glands of young rabbits was significantly greater than in the  the thymus glands of the aged group. Proliferation of the lymphocyte cells in the thymus glands of the group I was significantly greater than in the thymus glands of group II.