Pengaruh Pemberian Estrak Pheretima Asperigillum terhadap perubahan Histopatologik IIeum , Vesiak fellea dan Lien pada tikus Balb/c yang diinfeksi Salmonella Typhirium

Tahun 2005 Volume 40 Nomor 1
Oleh : Awal prasetyo, MF. Deasy Gelu, Riana Yosefarta, Dimas Adi Nugroho, Trise Kurniasari

Latar Belakang : Pheretima Asperigillum (Ph. Asperigillum) mengandung zat antipiretik dan antimikroba berupa lumbrofebrine dan anti microbial – like peptide.
Penelitian ini bertujuan membuktikan adnya perbedaan gambaran histopatologik ileum, hepar, vesica felea dan lien pada mencit Balb/c yang diinfeksi Salmonella Typhimurim(S.typhi) dan diberi ekstrak Ph.aspergillium.

Metode : Penelitian post test only control design ,menggunakan 36 mencit Balb/c , dibagi tiga kelompok ; kelompok kontrol (I) kelompok II yang diinduksi S.typhi , kelompok III diinduksi S .typhi diberi 0.3 mg/hari ekstrak Ph.Aspergillum per sonde pada hari  kedua sampai keenam . pada hari  ke tujuh dilakukan terminasi dan permrosesan jaringan. Data dianalisis univariart dan dilakukan uji Kruskall Wallis, dilanjutkan uji Mann Whitney,

Hasil : Rerata total skor umum dan skhusus ileum, tertinggi pada kelompok II . Uji Kruskall Wallis terhadap skor umum dan khusus  p=0,00 , uji Mann Whitney kelompok II dan III terhadap variabel skor umum dan khusus p=0,00.Rerata skor histoparologik umum dan khusus hepar, tertinggi pada kelompok II, sedangkan uji Kruskall Wallis  skor umum dan khusus  p=00  , namun  untuk volume h, ar p=0,005. Uji Mann – Whitney volume hepar kelompok II dan III p=0,16. Rerata skor histologik umum dan khusus vesica fellea  , tertinggi pada kelompok II , terendah pada kelompok I , Uji Kruskall Wallis dan Mann – Whitney didapatkan p=0.00 . Rerata volume , skor histopalogik umum dan khusus limpha, tertinggi pada kelompok II. Uji Kruskall – Wallis volume limpha p=0,002. Skor umum p=0,009, skor khusus  p=0,012 . Uji Mann-Whitney antara kelompok II dan III terhadap veriabel volume p=0,002, skor umum p=0,001 dan khusus p=0,01

Simpulan :
Pemberian ekstrak Ph.Asperigillum 0.3 mg/hari selama lima hari bersamaan dengan induksi demam tifoid menimbulkan perbedaan bermakna padangambaran histopatlogik jaringan ileum , hepar , vesica fella dan lien.

The Effect of Pheretima aspergillum on the Histopatology Changes of ileum , hepar, vesika fellea and Lien of Balb/c mice infected with Salmonella Typhirium

Bakground: Eatrhworms extract Pheretima Asperigillum (Ph,Asperigillum) is known having bacteria or bacteriostic effects and containts active agents as antyseptic  .The aim of this study was prove the effect Pheretima Asperigillum to the histophatological pattern of the illeum , galbladder and spleen of Balb/c , which were infected Salmonela typhimurium (S. Typhi).

Method :
This study used the post only control design , with 36 Balb/c were divided into 2 three group.Group I was control. Group II was induced by S.typhi intraperitineally. Group III ws induced and added Ph. Asperigillum extract 0,3 mg/day from second until 6 days .On the day 7 , termination was done , liver and spleen volume were measured as well as the histology speciment were examined . data were analyzed by Kruskall Wallis test and Mann- test for differences.

Result :
The group II showed the highest mean histology score of ileum. The Kruskal Walis test for general and specific score p=0,00. The Mann-whitney test between groupII and III general and specific score p=0,00.. The group II had volume  p=0,05. The Mann- Witney tes between group II and III in general and specific score p=0,00. and the Mann-Whitney test for volume p=0,16. The highest mean of general and specific score of galbladder at group II but the lowest at group I . the Kruskall-Wallis test in general and specific score p=0,00. but the Mann – Whitney test between group II and III p=0,00 .The Group II had highest mean of spleen volume , general and specific cores. The Kruskall – Waslis test in volume , =0,002 general score p= 0,009 , and specific score p=0,012. The Mann- Whitney test between Group II and III for volume p=0,02 , general score p=0,009, and specifis score p=0,012

Conclusion :
The administration Ph,Asperigillum extract at dose 0,3 mg/day orraly for five days have signifficant effect on histologycal changes of the illeum , liver,albladder and spleen.