Pengaruh Diet Kuning Telur Omega-3® dan Ayam Ras terhadap Ekspresi Sel Busa di Arteri Abdominalis Studi Eksperimental pada Tikus Wistar
Tahun 2002 Volume 37 Nomor 1
Oleh : Kiara Sinabutar, Awal Prasetyo
Latar belakang: Aterosklerosis ditengarai sebagai penyebab terjadinya penyakit-penyakit pembuluh darah dan penyakit jantung. Berbagai upava baik dietetik maupun medika mentosa dilakukan untuk mencegah terjadinya aterosklerosis. Asam lemak Omega-3 dilaporkan dapat menurunkan kadar kolesterol LDL yang bertanggung jawab terhadap terjadinya set-sel busa yang akan berlanjut ke arah siderosis pembuluh darah.
Metoda: Penelitian ini adalah penelitian eksperimental dengan rancangan The Post Test Only Group . Jumlah sanipel 8 ekor tikus Wistar dengan kriteria tertentu. dibagi menjadi 2 kelompok secara acak Kelompok / diinjeksi adrenalin i. v dan diberi diet kuning telur ras intermitten, sedangkan kelompok 2 diberi injeksi adrenalin i.v dan diet kuning telur Omega-3® .intermitten Pada han ke- 15 tikus didekapitasi untuk diperiksa jumlah sel busa di tunika intima arteri abdominalisnya. Dibuat 3 preparat aorta abdominalis pada tiap ekor tikus.
Hasil: Rerata jumlah sel busa di zon atunika intima aorta abdominalis di kelompok penerima diet kuning telur Omega-3® secara bermakna jauh iebih sedikit daripada ke!ompok penerima diet kuning telur biasa (2,5 ± 1.17 dan 9.0 ± 2,22. p 0.000)
Kesimpulan: Tikus yang diinjeksi adrenalin i.v dan diberi diet kuning telur Omega- 3 intermitten menunjukkan jumlah sel busa yang lebih sedikit daripada yang dimnjekci adrenalin iv dan diberi diet kuning telur ras intermitten.
Background: Atherosclerosis has been ident led as an important factor in the development of vascular and heart diseases. Many efforts either dietetics or medicamentous are being introduced to prevent the development of atherosclerosis. Unsaturated fatly acid known as Omega-3 been reported to reduce LDL-cholesterol that is responsible for the early development of foam cells.
Method: A post-test only group design was used in the study involving 8 Wistar, that were divided randomly into 2 groups. One group received adrenalin injection intravenously plus intermitten diet with regular egg yolk The second group received adrenalin injection intravenously plus intermitten die! with egg yolk containing Omega-3’ On the 15” days. rats were decapitated and from each rats 3 specimen were taken from the tunica inuima of aorta abdominal.
Result: The mean number of foam cells in tunica intima of aorta abdominalis in group receiving intermitten diet of egg yolk containing Omega-3 was significantly lower than those in the group receiving intermitten regular egg yolk (2.5 ± 1,17 and 9,0 1 ± 2,22 p= 0,000.).
Conclusion: The foam cells in tunica intima of aorta abdominalis of rats injected adrenalin intravenously given internmitten diet of egg yolk egg containing Omega-3 were less than those receiving intermnitten diet of regular egg yolk.