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Latar Belakang: Daun teh hijau memiliki kandungan senyawa Polifenol , yaitu: epigagallocacethin gallate(EGCg), epigallocacethin (EGC) dan cathethin lainnya. Penelitian terdahulu telah melaporkan bahwa polifenl teh dapat menginduksi sistem imun, termasuk :fagositosis, sekresi IFN-ץ dan sekresi IL – 12 Makrofag . Study ini bertujuan untuk membuktiakn bahwa polifenol teh hijau dan komponen aktifnya dapat meningkatkan kemampuan makrofag membunuh kuman dengan mengukur kadar nitrite oxide (NO)dan reactive oxigen Intermediate (ROI) pada kultur makrofag, dan menghitung jumlah bakteri dalam hati.

Metode: Desain penelitian ini adalh study eksperimental dengan the post test onli control group design . Mencit BALB/c dibagi secar acak menjadi 11 kelompok (tiap kelompok terdiri dari 6 ekor : kelompok pertama tidak diberi teh dan L .monocytogeneses, kelompok ke 2 diinokulasi L.Monocytogeneses tetapi tidak diberi teh , kelompok ke 3 s/d ke 5 diberi polyphenon -60 dengan dosis berbeda (1,5 :3 dan 6 mg/hari ) kelompok ke 6 s/d 8 diberi EGCg dengan dosis berbada (0,5;1 dan 2 mg/hari ) dan kelompok ke-9 s/d  11 diberi EGCg dengan dosis berbeda (0,15 ; 0,3 dan 0,6 mg/hari), mencit kelompok ke -3 s/d 11 mendapat perlakuan tersebut selama 14 hari diinokulasi L.Monocytogeneses pada hari ke 10
mencit dibunuh pada hari ke 15 untuk mengukur kadar NO dan ROI , serta menghitung jumlah bekteri dari jaringan hati.

Hasil:EGCg (0,5 ; 1 dan 2 mg/hari), (EGC(0,3 dan 0,6 mg/hari) , dan polyphenon-60(1,5;3 dan 6 mg/hari) meningkatkan kadar NO(p<0,05) dan menurunkan jumlah bakteri dalm hati (p<0,05). polyphenon-60(6 mg per hari) meningkatkan kadar ROI(p<0,05)

Background: Green Tea contains polyphenols compound that consist of epigallocacethin gallate (EGCg), epigallocacethin (EGC) , and their epimers . It was reported that Tea polyphenols could induce the immune system including phagocytosis, secretion of IFN-ץ and secretion of IL -12 by machrophages. The Aim of this study was to prove that tea polyphenols and its active component could increase the killing bacteria activity of macrophages by measuring the level of nitrite oxide (NO) and reactive oxygen intermediate (ROI) secreted by machrophages, and bacterial count the liver.

Methods: This study was an experimental research with post test only control group design . A total of 66 BALB/c were divided randomly into 11group : the 1^st group was not treated with tea polyphenols nor L. monocytogeneses , the 2^nd group was inoculated with L.monocytogeneses but not treated with tea polyphenols, 3^rd to 5^th group were  treated with tea plyphenon -60 in different doses (1,5 ; 3 and 6 mg/day), the 6^th to 8^th group were treated with EGCg  in diferent doses (0,5 ;1  and 2 mg/day) and the 9^th to 11^th group were treated with EGCg in different doses (0.15 ;0,3 and 0.6 mg/day) . the mice in the 3^rd to 11^th groups were treated for 14 days and they were inoculted with L.monocytogeneses at day 10^th . The mice were sacrified on the 15^th day for measuring the level of NO and ROI secreted by peritoneal macrophages , and counting becteria from the liver

Result: EGCg (0,5 ; 1 dan 2 mg/day), (EGC(0,3 dan 0,6 mg/day) , and polyphenon-60(1,5;3 dan 6 mg/day) increased the level of NO secreted by macrophages (p<0,05) and decreased the number of bacteria in the liver (p<0,05). polyphenon-60(6 mg /day) increased the levels of ROI secreted by macrophages (p<0,05).

Conclusion: Green Tea polyphenols and its active component increased the secretion of NO and ROI by macrophages , and decreased the number of bacteria in the liver