Efek Ekstrak Teh Hijau terhadap Uji Fagositosis dan Produksi NO pada Fagosit Manusia
Tahun 2005 Volume 40 Nomor 3
Oleh : Andrew Johan, Neni Susilaningsih
Latar belakang: Lekosit melakukan fagositosis untuk menelan mikroba. Kuman tersebut lebih cepat dibunuh dalam makrofag yang memproduksi NO dengan rangsangan IFN- dan TNF-a. Penelitian terdahulu melaporkan teh hijau me ningkatkan produksi sitokin tersebut. Penelitian in! dirancang dengan hipotesis bahwa teh hi meningkatkan fagositosis dan produksi NO pada fagosit dan manusia.
Metode: Rancangan penelitian in! adalah randomized pre and post test design pada sukarela wan dewasa, den gan jumlah subjek 16 orang . Uji aktivitas fagositosis dan produksi NO dilakukan sebelum subjek meminum teh h,jau dan sesudah subjek meminum 350 mg ekstrak teh hijau 2 Xlhari sehari selama 4 minggu. Fagosit diperoleh dan darah vena yang disentrifugasi dengan larutan Ficol-Isopaque. Aktivitas fagositos diukur dengan menghitungjumlah latex beads dalam 100 fagosit. Kadar NO dalam supernatan dan fagosit diukur den gan cara Griess Reaction Assay. Data dianalisis dengan up T apabila data berdistribusi normal, dan Wilcoxon signed ranks test apabila data tidak berdistri bus! normal.
Hasil: Rerata hasil aktivitas fagositosis sebelum dan sesudah subjek meminum ekstrak teh hi/au adalah 101,1 ± 7,9 dan 130,6 ± 14,3. Rerata kadar NO sebelum dan sesudah subjek meminum ekstrak teh hi/au adalah 34,3 ± 4,5 uM dan 144,3 ±76.6uM.
Simpulan: Aktivitas fagositosis dan produksi NO pada fagosit meningkat setelah subjek meminum 350mg ekstrak teh hijau 2 X/hari sehari selama 4 minggu (p<0, 05).
The Effects of Green Tea Extract on Phagocytosis Activity and Nitric Oxide Production in Human Phagocytes
Background: Phagocytosis is performed by leucocytes to engulf microorganisms. Intracellular killing of microbes enhanced in macrophages activated by IFN-y and TNF-a that induced NO production. Previous studies proved that green tea extract (GTE) significantly up-regulated the production of TNF-ci and IFN-y. This study was designed with the hypothesis that GTE act! vated phagocytosis and NO production in human phagocytes.
Methods: The study was constructed as a randomized pre and post test design with the participation of 16 adult volunteers. On the first day of the study, the samples of blood were intravenously taken from all the participants in separate tubes. In order to know the effect of the GTE on phagocytosis activity and NO production in human phagocytes, all the participants consumed 350mg GTE twice daily for 4 weeks. The phagocytosis activity was measured by counting the number of latex beads found in 100 phagocytes. The concentration of NO was quantified by the accumulation of nitrite by using Griess Reaction Assay. Statistical analysis were performed with T test for normally distributed data and Wilcoxon signed ranks test for data that were not normally distributed using SPSS 10 program.
Results: The phagocytosis activity before and after treated with GTE were 101.1± 7.9 and 130.6 ± 14.3 respectively. The NO production before and after treated with GTE were 34.3 ± 4.5 uM and 144.3 ± 76.6 uM respectively.
Conclusion: Phagocytosis activity and NO production increased significantly after the consumption of 350 mg GTE twice daily for 4 weeks (p<0, 05).